Bottery, Michael John orcid.org/0000-0001-5790-1756, Wood, Andrew James orcid.org/0000-0002-6119-852X and Brockhurst, Michael Alan orcid.org/0000-0003-0362-820X (2019) Temporal dynamics of bacteria-plasmid coevolution under antibiotic selection. The ISME Journal. pp. 559-562. ISSN 1751-7362
Abstract
Horizontally acquired genes can be costly to express even if they encode useful traits, such as antibiotic resistance. We previously showed that when selected with tetracycline, Escherichia coli carrying the tetracycline-resistance plasmid RK2 evolved mutations on both replicons that together provided increased tetracycline resistance at reduced cost. Here we investigate the temporal dynamics of this intragenomic coevolution. Using genome sequencing we show that the order of adaptive mutations was highly repeatable across three independently evolving populations. Each population first gained a chromosomal mutation in ompF which shortened lag phase and increased tetracycline resistance. This was followed by mutations impairing the plasmid-encoded tetracycline efflux pump, and finally, additional resistance-associated chromosomal mutations. Thus, reducing the cost of the horizontally acquired tetracycline resistance was contingent on first evolving a degree of chromosomally encoded resistance. We conclude therefore that the trajectory of bacteria-plasmid coevolution was constrained to a single repeatable path.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2018 |
Keywords: | Adaptation, Physiological/drug effects,Biological Coevolution,Escherichia coli/drug effects,Gene Transfer, Horizontal,Plasmids/genetics,Replicon,Selection, Genetic,Tetracycline Resistance/genetics,Tetracycline/pharmacology |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Mathematics (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 17 Sep 2018 16:20 |
Last Modified: | 18 Dec 2024 00:11 |
Published Version: | https://doi.org/10.1038/s41396-018-0276-9 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s41396-018-0276-9 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:135629 |
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