Gonzalez-Carter, DA, Ong, ZY orcid.org/0000-0001-8666-4382, McGilvery, CM et al. (3 more authors) (2019) L-DOPA functionalized, multi-branched gold nanoparticles as brain-targeted nano-vehicles. Nanomedicine: Nanotechnology, Biology and Medicine, 15 (1). pp. 1-11. ISSN 1549-9634
Abstract
The blood–brain barrier (BBB) is a protective endothelial barrier lining the brain microvasculature which prevents brain delivery of therapies against brain diseases. Hence, there is an urgent need to develop vehicles which efficiently penetrate the BBB to deliver therapies into the brain. The drug L-DOPA efficiently and specifically crosses the BBB via the large neutral amino acid transporter (LAT)-1 protein to enter the brain. Thus, we synthesized L-DOPA-functionalized multi-branched nanoflower-like gold nanoparticles (L-DOPA-AuNFs) using a seed-mediated method involving catechols as a direct reducing-cum-capping agent, and examined their ability to cross the BBB to act as brain-penetrating nanovehicles. We show that L-DOPA-AuNFs efficiently penetrate the BBB compared to similarly sized and shaped AuNFs functionalized with a non-targeting ligand. Furthermore, we show that L-DOPA-AuNFs are efficiently internalized by brain macrophages without inducing inflammation. These results demonstrate the application of L-DOPA-AuNFs as a non-inflammatory BBB-penetrating nanovehicle to efficiently deliver therapies into the brain.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Elsevier Inc. This is an author produced version of a paper published in Nanomedicine: Nanotechnology, Biology and Medicine. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Brain targeting; Blood–brain barrier; Gold nanoparticles; L-DOPA |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Sep 2018 12:39 |
Last Modified: | 03 Sep 2019 00:43 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.nano.2018.08.011 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:135378 |
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Filename: Manuscript revision submission Nanomedicine.pdf
Licence: CC-BY-NC-ND 4.0