Interactions between callose and cellulose revealed through the analysis of biopolymer mixtures

Abstract

The properties of (1,3)-β-glucans (i.e., callose) remain largely unknown despite their importance in plant development and defence. Here we use mixtures of (1,3)-β-glucan and cellulose, in ionic liquid solution and hydrogels, as proxies to understand the physico-mechanical properties of callose. We show that after callose addition the stiffness of cellulose hydrogels is reduced at a greater extent than predicted from the ideal mixing rule (i.e., the weighted average of the individual components’ properties). In contrast, yield behaviour after the elastic limit is more ductile in cellulose-callose hydrogels compared with sudden failure in 100% cellulose hydrogels. The viscoelastic behaviour and the diffusion of the ions in mixed ionic liquid solutions strongly indicate interactions between the polymers. Fourier-transform infrared analysis suggests that these interactions impact cellulose organisation in hydrogels and cell walls. We conclude that polymer interactions alter the properties of callose-cellulose mixtures beyond what it is expected by ideal mixing.

Metadata

Item Type:	Article
Authors/Creators:	Abou-Saleh, RH https://orcid.org/0000-0002-8471-2659 Hernandez-Gomez, M https://orcid.org/0009-0000-1603-7527 Amsbury, S https://orcid.org/0000-0002-2767-9768 Paniagua, C https://orcid.org/0000-0002-3263-1996 Bourdon, M https://orcid.org/0000-0001-7243-5642 Miyashima, S https://orcid.org/0000-0001-8267-3978 Helariutta, Y https://orcid.org/0000-0002-7287-8459 Fuller, M Budtova, T https://orcid.org/0000-0003-1835-2146 Connell, SD https://orcid.org/0000-0003-2500-5724 Ries, ME https://orcid.org/0000-0002-8050-3200 Benitez-Alfonso, Y https://orcid.org/0000-0001-9779-0413
Copyright, Publisher and Additional Information:	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0
Dates:	Accepted: 21 September 2018 Published (online): 31 October 2018 Published: 31 October 2018
Institution:	The University of Leeds
Academic Units:	The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Soft Matter Physics (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds)
Funding Information:	Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) EF/M027740/1 Leverhulme Trust Not Known
Depositing User:	Symplectic Publications
Date Deposited:	03 Sep 2018 12:58
Last Modified:	17 Dec 2024 09:49
Status:	Published
Publisher:	Springer Nature
Identification Number:	10.1038/s41467-018-06820-y
Related URLs:	Dataset
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:134699