Macrae, FL orcid.org/0000-0002-7092-0422, Duval, C orcid.org/0000-0002-4870-6542, Papareddy, P et al. (13 more authors) (2018) A fibrin biofilm covers blood clots and protects from microbial invasion. Journal of Clinical Investigation, 128 (8). pp. 3356-3368. ISSN 0021-9738
Abstract
Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining clots in human and mouse models. We demonstrated that only fibrin is required for formation of the film, and that it occurred in vitro and in vivo. The fibrin film connected to the underlying clot network through tethering fibers. It was digested by plasmin, and formation of the film was prevented with surfactants. Functionally, the film retained blood cells and protected against penetration by bacterial pathogens in a murine model of dermal infection. Our data show a remarkable aspect of blood clotting in which fibrin forms a protective film covering the external surface of the clot, defending the organism against microbial invasion.
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Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Macrae et al. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Funding Information: | Funder Grant number British Heart Foundation RG/13/3/30104 British Heart Foundation PG/16/60/32292 |
Depositing User: | Symplectic Publications |
Date Deposited: | 16 Aug 2018 10:05 |
Last Modified: | 16 Aug 2018 10:05 |
Status: | Published |
Publisher: | American Society for Clinical Investigation |
Identification Number: | 10.1172/JCI98734 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:134632 |
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