Tes, D., Kratkiewicz, K., Aber, A. et al. (5 more authors) (2018) Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study. Biomedical Engineering and Computational Biology, 9. ISSN 1179-5972
Abstract
Alzheimer disease is the most common form of dementia, affecting more than 5million people in the United States. During the progression of Alzheimer disease, a particular protein begins to accumulate in the brain and also in extensions of the brain, ie, the retina. This protein, amyloid-β (Aβ), exhibits fluorescent properties. The purpose of this research article is to explore the implications of designing a fluorescent imaging system able to detect Aβ proteins in the retina. We designed and implemented a fluorescent imaging system with a range of applications that can be reconfigured on a fluorophore to fluorophore basis and tested its feasibility and capabilities using Cy5 and CRANAD-2 imaging probes. The results indicate a promising potential for the imaging system to be used to study the Aβ biomarker. A performance evaluation involving ex vivo and in vivo experiments is planned for future study.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
Keywords: | Fluorescence imaging; Cy5; CRANAD-2; amyloid-β; Alzheimer disease; early diagnosis; instrumentation |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Health and Related Research (Sheffield) > ScHARR - Sheffield Centre for Health and Related Research |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 Aug 2018 12:26 |
Last Modified: | 14 Aug 2018 04:02 |
Published Version: | https://doi.org/10.1177%2F1179597218781081 |
Status: | Published |
Publisher: | SAGE Publications |
Refereed: | Yes |
Identification Number: | 10.1177/1179597218781081 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:134515 |