Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19

Abstract

It has previously been reported that human adipose-derived stem cells (hASCs) can promote the regeneration of damaged tissues in rats with liver failure through a 'paracrine effect'. Here we demonstrate a therapeutic effect of hASCs derived Extracellular Vesicles (EVs) on rat models with acute liver failure, as shown by the improvement of the survival rate by >70% compared to controls. Gene sequencing of rat liver revealed an increase in human long-chain non-coding RNA (lncRNA) H19 after hASC-derived EVs transplantation. When the H19 coding sequence was silenced in hASCs and EVs were then collected for treatment of rats with liver failure, we saw a decrease in the survival rate to 40%, compared to treatment with EVs generated from non-silenced hASCs. These data indicate that lncRNA H19 may be a potential therapeutic target for the treatment of liver failure.

Metadata

Item Type:	Article
Authors/Creators:	Jin, Y. Wang, J. Li, H. Gao, S. Shi, R. Yang, D. Wang, X. Wang, X. Zhu, L. Wang, X. Chen, C. Ning, K. https://orcid.org/0000-0002-0771-1134 Fu, Q. Xu, J. Gao, Z.
Copyright, Publisher and Additional Information:	© 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords:	Adipose-derived stem cells; Extracellular vesicles; Liver failure; LncRNA; Regeneration
Dates:	Published: August 2018 Published (online): 2 August 2018 Accepted: 12 July 2018
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Sheffield Teaching Hospitals
Depositing User:	Symplectic Sheffield
Date Deposited:	14 Aug 2018 10:46
Last Modified:	27 Aug 2020 10:11
Status:	Published
Publisher:	Elsevier
Refereed:	Yes
Identification Number:	10.1016/j.ebiom.2018.07.015
Related URLs:	PubMed URL
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:134486

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Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19

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