Chittock, J. orcid.org/0000-0002-1595-7441, Brown, K., Cork, M.J. et al. (1 more author) (2018) Quantification of natural moisturizing factors at the skin surface using a portable infrared spectrometer device: a pilot, calibration model. In: British Journal of Dermatology. The 10th George Rajka International Symposium on Atopic Dermatitis, 11-13 Apr 2018, Utrecht, Netherlands. Wiley , E35-E36.
Abstract
Attenuated total reflectance Fourier transform infrared spectroscopy (FTIR) is a useful technique for the molecular analysis of surfaces, including the skin, with promising translational clinical potential. Skin‐surface levels of natural moisturizing factor (NMF) are a biomarker of filaggrin (FLG) status (both inherited and acquired) and skin dryness. FLG‐related atopic dermatitis (AD) is associated with more severe and persistent disease. The objective of this study was to combine FTIR with chemometric analysis to generate a pilot calibration model for the in vivo quantification of NMF at the skin surface using a portable FTIR device. This study was performed in a climate‐controlled, skin barrier suite located at the University of Sheffield, U.K. patients with either healthy skin or AD were recruited from the local Sheffield community, and informed consent was obtained prior to enrolment in the study. A diagnosis of AD was made using the U.K. working party criteria, and disease severity was classified by the Eczema Area and Severity Index score. Genotyping for the five most common European loss‐of‐function FLG mutations was performed from buccal swabs. FTIR spectra of 4‐cm−1 resolution were collected from the volar forearm and antecubital fossa in conjunction with tape strips for the quantification of NMF components by high‐performance liquid chromatography (HPLC) and o‐phthaldialdehyde derivatization. Transepidermal water loss (TEWL) and stratum corneum hydration (SCH) measurements were collected as an assessment of barrier function. Ethical permission for this study was granted by the National Health Service Trent research ethics committee. Partial least‐squares regression modelling of absorbance in the mid infrared spectral region (1710–1185 cm−1) with skin‐surface NMF components determined by HPLC generated a predictive r2 value of 0.90. Modelling was superior on the antecubital fossa compared with the forearm, presumably due to the increased FTIR signal obtained from this site. Predicted NMF values correlated with FLG status, TEWL and SCH. FTIR combined with chemometric analysis is a suitable technique for the instantaneous in vivo quantification of NMF at the skin surface. The use of a portable FTIR device makes this methodology suitable for any clinical setting, with the potential to inform long‐term treatment strategies in AD.
Metadata
Item Type: | Proceedings Paper |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | This is the peer reviewed version of the following article: (2018), Abstracts of the 10th George Rajka International Symposium on Atopic Dermatitis, 11–13 April 2018, Utrecht, the Netherlands. Br J Dermatol, which has been published in final form at https://doi.org/10.1111/bjd.16718. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 22 Aug 2018 08:42 |
Last Modified: | 19 Dec 2022 13:50 |
Published Version: | https://doi.org/10.1111/bjd.16718 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1111/bjd.16718 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:134485 |