Raveendran, M orcid.org/0000-0003-3968-3673, Lee, AJ orcid.org/0000-0003-2268-4645, Wälti, C orcid.org/0000-0001-9286-5359 et al. (1 more author) (2018) Analysis of 2D DNA Origami with Nanopipettes. ChemElectroChem, 5 (20). pp. 3014-3020. ISSN 2196-0216
Abstract
Nanopipettes are a useful tool for the detection and analysis of biological molecules at the single‐molecule level. Here, we employ nanopipettes fabricated from glass capillaries to identify differently structured 2D DNA origami through the distinctive amplitude and dwell time of the ion current peak resulting from the translocation of the origami through the nanopipette pore. We demonstrate that the ion current peak originating from frame‐like DNA origami comprises two individual peaks in contrast to solid tiles of similar size that only lead to a single peak in the ion current. Interestingly, the fine details of the shape of the double‐peak characteristic of the translocation of DNA origami frames are correlated with the structural features of the DNA origami. In particular, the size of the central cavity governs the lag time between the two constituent peaks of the double‐peak. This work demonstrates the ability of glass nanopipettes to identify and characterize differently structured DNA origami of similar size, advancing the potential of nanopipettes for high‐sensitivity single‐molecule studies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Raveendran, M. , Lee, A. , Walti, C. and Actis, P. (2018), Analysis of 2‐D DNA origami with nanopipettes. ChemElectroChem, doi:10.1002/celc.201800732, which has been published in final form at https://doi.org/10.1002/celc.201800732. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | DNA nanotechnology; DNA origami; nanopipette; nanopore; single-molecule analysis |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 31 Jul 2018 14:39 |
Last Modified: | 06 Apr 2020 13:00 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/celc.201800732 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:133975 |