Wilson, Katherine M., Jagger, Alistair M, Walker, Matthew et al. (5 more authors) (2018) Glycans modify mesenchymal stem cell differentiation to impact on the function of resulting osteoblasts. Journal of Cell Science. jcs209452. ISSN 0021-9533
Abstract
Glycans are inherently heterogeneous, yet glycosylation is essential in eukaryotes, and glycans show characteristic cell type-dependent distributions. By using an immortalized human mesenchymal stromal cell (MSC) line model, we show that both N- and O-glycan processing in the Golgi functionally modulates early steps of osteogenic differentiation. We found that inhibiting O-glycan processing in the Golgi prior to the start of osteogenesis inhibited the mineralization capacity of the formed osteoblasts 3 weeks later. In contrast, inhibition of N-glycan processing in MSCs altered differentiation to enhance the mineralization capacity of the osteoblasts. The effect of N-glycans on MSC differentiation was mediated by the phosphoinositide-3-kinase (PI3K)/Akt pathway owing to reduced Akt phosphorylation. Interestingly, by inhibiting PI3K during the first 2 days of osteogenesis, we were able to phenocopy the effect of inhibiting N-glycan processing. Thus, glycan processing provides another layer of regulation that can modulate the functional outcome of differentiation. Glycan processing can thereby offer a novel set of targets for many therapeutically attractive processes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018. Published by The Company of Biologists Ltd. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details |
Keywords: | Glycan processing,Hydroxyapatite,Kifunensine,Osteogenesis,PI3K signalling |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Physics (York) |
Funding Information: | Funder Grant number EPSRC EP/I001514/1 |
Depositing User: | Pure (York) |
Date Deposited: | 27 Jul 2018 09:20 |
Last Modified: | 08 Feb 2025 00:26 |
Published Version: | https://doi.org/10.1242/jcs.209452 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1242/jcs.209452 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:133900 |