Harris, S., Boorman, L., Das, D. et al. (6 more authors) (2018) Physiological and pathological brain activation in the anesthetized rat produces hemodynamic-dependent cortical temperature increases that can confound the BOLD fMRI signal. Frontiers in Neuroscience, 12. 550. ISSN 1662-4548
Abstract
Anesthetized rodent models are ubiquitous in pre-clinical neuroimaging studies. However, because the associated cerebral morphology and experimental methodology results in a profound negative brain-core temperature differential, cerebral temperature changes during functional activation are likely to be principally driven by local inflow of fresh, core-temperature, blood. This presents a confound to the interpretation of blood-oxygenation level-dependent (BOLD) functional magnetic resonance imaging (fMRI) data acquired from such models, since this signal is also critically temperaturedependent. Nevertheless, previous investigation on the subject is surprisingly sparse. Here, we address this issue through use of a novel multi-modal methodology in the urethaneanesthetizedrat.Werevealthatsensorystimulation,hypercapniaandrecurrent acute seizures induce significant increases in cortical temperature that are preferentially correlated to changes in total hemoglobin concentration (Hbt), relative to cerebral blood flow and oxidative metabolism. Furthermore, using a phantom-based evaluation of the effect of such temperature changes on the BOLD fMRI signal, we demonstrate a robust inverse relationship between both variables. These findings suggest that temperature increases, due to functional hyperemia, should be accounted for to ensure accurate interpretation of BOLD fMRI signals in pre-clinical neuroimaging studies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Harris, Boorman, Das, Kennerley, Sharp, Martin, Redgrave, Schwartz and Berwick. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. http://creativecommons.org/licenses/by/4.0/ |
Keywords: | corticaltemperature; cerebral metabolic rate of oxygen; cerebral hemodynamics; sensory stimulation; hypercapnia; seizures; BOLD fMRI |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Psychology (Sheffield) |
Funding Information: | Funder Grant number EPILEPSY RESEARCH UK UNSPECIFIED MEDICAL RESEARCH COUNCIL MR/M013553/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Jul 2018 11:11 |
Last Modified: | 01 Oct 2018 14:04 |
Published Version: | https://doi.org/10.3389/fnins.2018.00550 |
Status: | Published |
Publisher: | Frontiers Media |
Refereed: | Yes |
Identification Number: | 10.3389/fnins.2018.00550 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:133475 |