Gallego-Giraldo, L, Posé, S, Pattathil, S et al. (10 more authors) (2018) Elicitors and defense gene induction in plants with altered lignin compositions. The New phytologist, 219 (4). pp. 1235-1251. ISSN 0028-646X
Abstract
A reduction in the lignin content in transgenic plants induces the ectopic expression of defense genes, but the importance of altered lignin composition in such phenomena remains unclear.
Two Arabidopsis lines with similar lignin contents, but strikingly different lignin compositions, exhibited different quantitative and qualitative transcriptional responses. Plants with lignin composed primarily of guaiacyl units overexpressed genes responsive to oomycete and bacterial pathogen attack, whereas plants with lignin composed primarily of syringyl units expressed a far greater number of defense genes, including some associated with cis‐jasmone‐mediated responses to aphids; these plants exhibited altered responsiveness to bacterial and aphid inoculation.
Several of the defense genes were differentially induced by water‐soluble extracts from cell walls of plants of the two lines. Glycome profiling, fractionation and enzymatic digestion studies indicated that the different lignin compositions led to differential extractability of a range of heterogeneous oligosaccharide epitopes, with elicitor activity originating from different cell wall polymers.
Alteration of lignin composition affects interactions with plant cell wall matrix polysaccharides to alter the sequestration of multiple latent defense signal molecules with an impact on biotic stress responses.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2018 The Authors, New Phytologist (c) 2018 New Phytologist Trust. This is the pre-peer reviewed version of the following article: Gallego-Giraldo, L, Posé, S, Pattathil, S et al. (2018) Elicitors and defense gene induction in plants with altered lignin compositions. The New phytologist. which has been published in final form at https://doi.org/10.1111/nph.15258. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
Keywords: | Arabidopsis thaliana; cell wall remodeling; damage‐associated molecular patterns; defense response genes; genetic modification; glycome profiling; lignin composition |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Jul 2018 11:14 |
Last Modified: | 27 Jun 2019 00:39 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/nph.15258 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:133174 |