Marsay, K., Roehl, H., Monk, P. orcid.org/0000-0003-4637-3059 et al. (2 more authors) (2017) Tetraspanins in zebrafish development. In: Mechanisms of Development. 18th International Congress of Developmental Biology, 18-22 Jun 2017, Singapore. Elsevier , S64-S65.
Abstract
Introduction: Tetraspanins represent a family of integral membrane proteins involved in cell-cell interaction, including adhesion, fusion, differentiation and proliferation. These basic functions are essential for embryonic development, yet there is little research on the role of tetraspanins in this process. The aim of my project is to pilot zebrafish as a new and sensitive model for assessing tetraspanin function in vertebrate development.
Background: There are approximately 50 tetraspanin genes in zebrafish, representing orthologues of most of the 33 mammalian genes. mRNA expression analysis has shown that at least 22 of these are expressed in zebrafish embryos and thus may regulate developmental processes. CD9 is a well-characterized tetraspanin and we have shown that zebrafish CD9 orthologues are present in the posterior lateral line (pLL), a sensory system comprised of hair-cell containing neuromasts. The development of the pLL coordinates proliferation, deposition and migration simultaneously and thus requires highly regulated cell interactions.
Major findings: We generated CRISPR double knockouts (dKOs) of both zebrafish CD9 paralogues. The dKOs are adult viable and fertile, in contrast to mouse CD9 KO females which are sterile. Inspection of the pLL in the CD9 KOs revealed that there is measurably slower migration of the primordium and fewer hair cells in the posterior neuromasts at 10 dpf. Furthermore we observed a reduced regenerative capacity of the dKO neuromasts, and also upregulation of CD9 paralogues during bone repair.
Conclusions: Our results suggest a role for CD9 in collective cell migration and hair cell development. We will analyse the organisation and migration of the primordium in more detail as well as the development and regeneration of the neuromasts and bone. This will be aided by generating fluorescent transgenic zebrafish to visualise dynamic processes involved. This offers a unique insight into the in vivo function of tetraspanins.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Elsevier. This is an author produced version of a paper subsequently published in Mechanisms of Development. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Jun 2018 09:55 |
Last Modified: | 19 Dec 2022 13:49 |
Published Version: | https://doi.org/10.1016/j.mod.2017.04.142 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.mod.2017.04.142 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:132195 |