Suchanova, B orcid.org/0000-0003-0047-0013 and Tuma, R (2008) Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry. Microbial Cell Factories, 7 (12). 12. ISSN 1475-2859
Abstract
Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2008 Suchanova and Tuma; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | CYTOCHROME-C; hydrogen/deuterium exchange; CONFORMATIONAL-CHANGES; POLYMERASE COMPLEX; ION-CYCLOTRON-RESONANCE; TRANSFORM INFRARED-SPECTROSCOPY; SINGLE-POINT MUTATION; RNA PACKAGING MOTOR; PROTEIN-STRUCTURE; STRUCTURAL-CHANGES; Energy Landscape; Supercritical Fluid Chromatography; Ultra Performance Liquid Chromatography; Folding Intermediate; Deuterium Incorporation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Aug 2019 14:18 |
Last Modified: | 25 Jun 2023 21:22 |
Status: | Published |
Publisher: | BMC |
Identification Number: | 10.1186/1475-2859-7-12 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:131830 |