Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models

Abstract

Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.

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Item Type:	Article
Authors/Creators:	Allocca, G. Kusumbe, A.P. Ramasamy, S.K. Wang, N. https://orcid.org/0000-0002-2663-7515
Copyright, Publisher and Additional Information:	© 2016 Nature Publishing Group. This is an author produced version of a paper subsequently published in BoneKEy Reports. Uploaded in accordance with the publisher's self-archiving policy.
Dates:	Published: 7 December 2016 Published (online): 7 December 2016 Accepted: 2 November 2016
Institution:	The University of Sheffield
Depositing User:	Symplectic Sheffield
Date Deposited:	22 May 2018 14:03
Last Modified:	23 May 2018 09:02
Published Version:	https://doi.org/10.1038/bonekey.2016.84
Status:	Published
Publisher:	Nature Publishing Group
Refereed:	Yes
Identification Number:	10.1038/bonekey.2016.84
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:130910

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Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models

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