Raïf, EM (2008) Effect of cyclic tensile load on the regulation of the expression of matrix metalloproteases (MMPs ‐1, ‐3) and structural components in synovial cells. Journal of Cellular and Molecular Medicine, 12 (6A). pp. 2439-2448. ISSN 1582-1838
Abstract
Synovial cells are reported to colonize synthetic ligament scaffolds following anterior cruciate ligament (ACL) reconstruction but the process leading to ligamentization is poorly understood. The present study investigated the effect of cyclic tensile strain on the expression of genes involved in matrix remodelling in bovine synovial cells seeded onto an artificial ligament scaffold. Synovial cells were seeded and cultured on polyester scaffolds for 3 weeks and subsequently subjected to cyclic tensile strain of 4.5% for 1 hr at frequency of 1 Hz. Changes in the levels of expression of genes for major ligament components (type I and type III collagen) and also metalloproteinases (MMP‐1 and MMP‐3), and TIMP‐1 were examined using RT‐PCR. Additionally, metalloproteinase activity was measured using both zymography and collagenase assays. The gene expression of MMP‐3 transcripts in the loaded group was almost 3‐fold that observed in control group but no differences were observed in other transcripts. Consistent with these findings, MMP‐3 activity increased by 85% under mechanical stimulus, and MMP‐1 activity showed no changes. Over expression of MMP‐3 under cyclic tensile load may mediate the proteolysis of certain substrates surrounding the ligament scaffold. This will play a critical role in facilitating cell migration, proliferation and tissue remodelling by breaking down the provisional tissue formed by the synovium, and by generating factors that induce angiogenesis and chemotactic cell migration.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2007 The Author Journal compilation © 2007 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License. |
Keywords: | ligaments; mechanical strain; matrix metalloproteinase; tissue engineering; synovial cells |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Aug 2019 15:01 |
Last Modified: | 25 Jun 2023 21:19 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/j.1582-4934.2008.00245.x |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:129992 |