Ismail, A, Levin, RE, Riaz, M et al. (3 more authors) (2017) Effect of different microbial concentrations on binding of aflatoxin M₁ and stability testing. Food Control, 73 (Part B). pp. 492-496. ISSN 0956-7135
Abstract
Aflatoxin M₁ (AFM₁) is a group 2b category carcinogenic compound of global concern due to its occurrence in milk. Various microbes have been employed for the binding of AFM₁ and to reduce its bioavailability in humans. In the current study three strains of lactic acid bacteria, a strain of Saccharomyces cerevisiae and a mixture of all four were used to evaluate their binding potentials for AFM₁. Milk samples were spiked with two different concentrations of AFM₁, 0.05 and 0.1 μg/l, and four concentrations of microbes, 10⁷, 10⁸, 10⁹ and 10¹⁰ cfu/ml, were tested to evaluate their binding potentials. The concentration of AFM₁ and microbes were found to significantly affect the binding potentials of microbes. Saccharomyces cerevisiae, Lactobacillus helveticus and the mixture of microbes at the concentration of 10¹⁰ cfu/ml resulted in 100% binding of AFM₁. Lactobacillus helveticus was found to have a higher binding potential than any other lactic acid bacteria reported previously. The binding of AFM₁ with microbes was reversible as the washing test resulted in ∼20–∼70% release of AFM₁. These results indicate that the microbes can be effectively used for the reduction of AFM₁ levels up to safe limits in milk and milk products.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Aflatoxin M1; Milk; ELISA; Binding; Washing |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 20 Apr 2018 11:19 |
Last Modified: | 20 Apr 2018 11:19 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.foodcont.2016.08.040 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:129877 |