Oates, A orcid.org/0000-0003-0519-4556, Lindsay, S, Mistry, H et al. (2 more authors) (2018) Modelling antisepsis using defined populations of facultative and anaerobic wound pathogens grown in a basally perfused biofilm model. Biofouling, 34 (5). pp. 507-518. ISSN 0892-7014
Abstract
An in vitro model was developed to assess the effects of topical antimicrobials on taxonomically defined wound biofilms. Biofilms were exposed over seven days to povidone-iodine, silver acetate or polyhexamethylene biguanide (PHMB) at concentrations used in wound dressings. The rank order of tolerance in multi-species biofilms, based on an analysis of the average bacterial counts over time was P. aeruginosa > methicillin-resistant Staphylococcus aureus (MRSA) > B. fragilis > S. pyogenes. The rank order of effectiveness for the antimicrobials in the biofilm model was povidone-iodine > PHMB > silver acetate. None of the test compounds eradicated P. aeruginosa or MRSA from the biofilms although all compounds except silver acetate eliminated S. pyogenes. Antimicrobial effectiveness against bacteria grown in multi-species biofilms did not correlate with planktonic susceptibility. Defined biofilm populations of mixed-species wound pathogens could be maintained in the basal perfusion model, facilitating the efficacy testing of treatments regimens and potential dressings against multi-species biofilms composed of wound isolates.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Biofouling on 06 Jun 2018, available online: http://www.tandfonline.com/10.1080/08927014.2018.1466115. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Wound biofilm; in vitro model; MRSA; anaerobic; antisepsis |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Healthcare (Leeds) > Nursing Adult (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Apr 2018 13:24 |
Last Modified: | 06 Jun 2019 00:42 |
Status: | Published |
Publisher: | Taylor & Francis |
Identification Number: | 10.1080/08927014.2018.1466115 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:129665 |