Montanari, E, Oates, A orcid.org/0000-0003-0519-4556, Di Meo, C et al. (8 more authors) (2018) Hyaluronan‐Based Nanohydrogels for Targeting Intracellular S. Aureus in Human Keratinocytes. Advanced Healthcare Materials, 7 (12). 1701483. ISSN 2192-2640
Abstract
Staphylococcus aureus is one of the most significant human pathogens that is frequently isolated in a wide range of superficial and systemic infections. The ability of S. aureus to invade and survive within host cells such as keratinocytes and host immune cells has been increasingly recognized as a potential factor in persistent infections and treatment failures. The incorporation of antibiotics into hyaluronan‐cholesterol nanohydrogels represents a novel paradigm in the delivery of therapeutic agents against intracellular bacteria. The work presented herein shows that NHs quickly enter human keratinocytes and accumulate into lysosomes. When used for targeting intracellular S. aureus the antimicrobial activity of loaded levofloxacin is enhanced, possibly changing the antibiotic intracellular fate from cytosol to lysosome. Indeed, gentamicin, an antibiotic that predominantly accumulates in lysosomes, shows significant and equal antibacterial activity when entrapped into NHs. These results strongly suggest that lysosomal formulations may display preferential activity toward intracellular S. aureus, opening new avenues for the use of HA‐based NHs for treatment of such skin infections.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: E. Montanari, A. Oates, C. Di Meo, J. Meade, R. Cerrone, A. Francioso, D. Devine, T. Coviello, P. Mancini, L. Mosca, P. Matricardi, Adv. Healthcare Mater. 2018, 7, 1701483. https://doi.org/10.1002/adhm.201701483, which has been published in final form at https://doi.org/10.1002/adhm.201701483. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | antibiotic delivery; hyaluronan; intracellular infections; keratinocytes; nanohydrogels |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Healthcare (Leeds) > Nursing Adult (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Apr 2018 13:00 |
Last Modified: | 25 Apr 2019 00:38 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/adhm.201701483 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:129558 |