Lee, SJ, Castro, ER, Guijt, RM et al. (2 more authors) (2017) Van de Graaff generator for capillary electrophoresis. Journal of Chromatography A, 1517. pp. 195-202. ISSN 0021-9673
Abstract
A new approach for high voltage capillary electrophoresis (CE) is proposed, which replaces the standard high voltage power supply with a Van de Graaff generator, a low current power source. Because the Van de Graaff generator is a current-limited source (10 μA), potentials exceeding 100 kV can be generated for CE when the electrical resistance of the capillary is maximized. This was achieved by decreasing the capillary diameter and reducing the buffer ionic strength. Using 2 mM borate buffer and a 5 μm i.d. capillary, fluorescently labeled amino acids were separated with efficiencies up to 3.5 million plates; a 5.7 fold improvement in separation efficiency compared to a normal power supply (NPS) typically used in CE. This separation efficiency was realized using a simple set-up without significant Joule heating, making the Van de Graaff generator a promising alternative for applying the high potentials required for enhancing resolution in the separation and analysis of highly complex samples, for example mixtures of glycans.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Elsevier B.V. This is an author produced version of a paper published in Journal of Chromatography A. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Van de Graaff generator; High Voltage capillary electrophoresis; High separation efficiency; High separation resolution |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Earth and Environment (Leeds) > Inst for Climate & Atmos Science (ICAS) (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 27 Mar 2018 10:15 |
Last Modified: | 12 Aug 2018 00:39 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.chroma.2017.08.026 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128988 |