Brabham, Robin Louis, Spears, Richard James, Walton, Julia et al. (3 more authors) (2018) Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification. Chemical Communications. pp. 1501-1504. ISSN 1364-548X
Abstract
Protein bioconjugation frequently makes use of aldehydes as reactive handles, with methods for their installation being highly valued. Here a new, powerful strategy to unmask a reactive protein aldehyde is presented. A genetically encoded caged glyoxyl aldehyde, situated in solvent-accessible locations, can be rapidly decade through treatment with just one equivalent of allylpalladium(II) chloride dimer at physiological pH. The protein aldehyde can undergo subsequent oxime ligation for site-selective protein modification. Quick yet mild conditions, orthogonality and powerful exposed reactivity make this strategy of great potential in protein modification.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number EPSRC EP/M028127/1 EPSRC EP/P030653/1 |
Depositing User: | Pure (York) |
Date Deposited: | 22 Mar 2018 09:30 |
Last Modified: | 17 Dec 2024 00:10 |
Status: | Published |
Refereed: | Yes |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128868 |
Download
Filename: C7CC07740H.pdf
Description: Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification
Licence: CC-BY 2.5