Hong, Huynh A., Ferreira, William T., Hosseini, Siamand et al. (7 more authors) (2017) The Spore Coat Protein CotE Facilitates Host Colonization by Clostridium difficile. The Journal of Infectious Diseases. pp. 1452-1459. ISSN 0022-1899
Abstract
Clostridium difficile infection (CDI) is an important hospital-acquired infection resulting from the germination of spores in the intestine as a consequence of antibiotic-mediated dysbiosis of the gut microbiota. Key to this is CotE, a protein displayed on the spore surface and carrying 2 functional elements, an N-terminal peroxiredoxin and a C-terminal chitinase domain. Using isogenic mutants, we show in vitro and ex vivo that CotE enables binding of spores to mucus by direct interaction with mucin and contributes to its degradation. In animal models of CDI, we show that when CotE is absent, both colonization and virulence were markedly reduced. We demonstrate here that the attachment of spores to the intestine is essential in the development of CDI. Spores are usually regarded as biochemically dormant, but our findings demonstrate that rather than being simply agents of transmission and dissemination, spores directly contribute to the establishment and promotion of disease.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author 2017 |
Keywords: | chitinase,Clostridium difficile,colonization,mucus,spores,virulence |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 21 Mar 2018 10:00 |
Last Modified: | 16 Oct 2024 14:34 |
Published Version: | https://doi.org/10.1093/infdis/jix488 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1093/infdis/jix488 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128823 |
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Filename: jix488.pdf
Description: The Spore Coat Protein CotE Facilitates Host Colonization by Clostridium difficile
Licence: CC-BY 2.5