Vale da Costa, B.L., Basso, T.O., Raghavendran, V. orcid.org/0000-0002-2021-2786 et al. (1 more author) (2018) Anaerobiosis revisited: growth of Saccharomyces cerevisiae under extremely low oxygen availability. Applied Microbiology and Biotechnology, 102 (5). pp. 2101-2116. ISSN 0175-7598
Abstract
The budding yeast Saccharomyces cerevisiae plays an important role in biotechnological applications, ranging from fuel ethanol to recombinant protein production. It is also a model organism for studies on cell physiology and genetic regulation. Its ability to grow under anaerobic conditions is of interest in many industrial applications. Unlike industrial bioreactors with their low surface area relative to volume, ensuring a complete anaerobic atmosphere during microbial cultivations in the laboratory is rather difficult. Tiny amounts of O2 that enter the system can vastly influence product yields and microbial physiology. A common procedure in the laboratory is to sparge the culture vessel with ultrapure N2 gas; together with the use of butyl rubber stoppers and norprene tubing, O2 diffusion into the system can be strongly minimized. With insights from some studies conducted in our laboratory, we explore the question ‘how anaerobic is anaerobiosis?’. We briefly discuss the role of O2 in non-respiratory pathways in S. cerevisiae and provide a systematic survey of the attempts made thus far to cultivate yeast under anaerobic conditions. We conclude that very few data exist on the physiology of S. cerevisiae under anaerobiosis in the absence of the anaerobic growth factors ergosterol and unsaturated fatty acids. Anaerobicity should be treated as a relative condition since complete anaerobiosis is hardly achievable in the laboratory. Ideally, researchers should provide all the details of their anaerobic set-up, to ensure reproducibility of results among different laboratories.
A correction to this article is available online at http://eprints.whiterose.ac.uk/131930/
https://doi.org/10.1007/s00253-018-9036-z
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Springer-Verlag GmbH Germany, part of Springer Nature. This is an author produced version of a paper subsequently published in Applied Microbiology and Biotechnology. Uploaded in accordance with the publisher's self-archiving policy. The final publication is available at Springer via https://doi.org/10.1007/s00253-017-8732-4. |
Keywords: | Anaerobiosis; Oxygen; Saccharomyces cerevisiae; Chemostat cultivation; Anaerobic growth factors |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 20 Mar 2018 13:36 |
Last Modified: | 03 Feb 2019 01:39 |
Published Version: | https://doi.org/10.1007/s00253-017-8732-4 |
Status: | Published |
Publisher: | Springer Verlag |
Refereed: | Yes |
Identification Number: | 10.1007/s00253-017-8732-4 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128754 |