Panou, M-M, Prescott, EL, Hurdiss, DL orcid.org/0000-0003-3834-5808 et al. (11 more authors) (2018) Agnoprotein Is an Essential Egress Factor during BK Polyomavirus Infection. International Journal of Molecular Sciences, 19 (3). 902. ISSN 1661-6596
Abstract
BK polyomavirus (BKPyV; hereafter referred to as BK) causes a lifelong chronic infection and is associated with debilitating disease in kidney transplant recipients. Despite its importance, aspects of the virus life cycle remain poorly understood. In addition to the structural proteins, the late region of the BK genome encodes for an auxiliary protein called agnoprotein. Studies on other polyomavirus agnoproteins have suggested that the protein may contribute to virion infectivity. Here, we demonstrate an essential role for agnoprotein in BK virus release. Viruses lacking agnoprotein fail to release from host cells and do not propagate to wild-type levels. Despite this, agnoprotein is not essential for virion infectivity or morphogenesis. Instead, agnoprotein expression correlates with nuclear egress of BK virions. We demonstrate that the agnoprotein binding partner α-soluble N-ethylmaleimide sensitive fusion (NSF) attachment protein (α-SNAP) is necessary for BK virion release, and siRNA knockdown of α-SNAP prevents nuclear release of wild-type BK virions. These data highlight a novel role for agnoprotein and begin to reveal the mechanism by which polyomaviruses leave an infected cell.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license http://creativecommons.org/licenses/by/4.0/). |
Keywords: | polyomavirus; agnoprotein; virus exit |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cryo EM, Image Processing (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Virology 2 (Leeds) |
Funding Information: | Funder Grant number Kidney Research UK ST4/2014 Wellcome Trust 105221/Z/14/Z MRC MR/K012665/1 Kidney Research UK RP25/2013 |
Depositing User: | Symplectic Publications |
Date Deposited: | 20 Mar 2018 14:09 |
Last Modified: | 25 Jun 2023 21:16 |
Status: | Published |
Publisher: | MDPI |
Identification Number: | 10.3390/ijms19030902 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128624 |