Bastan, R., Eskandari, N., Ardakani, H.J. et al. (1 more author) (2017) Effects of fostriecin on β2-adrenoceptor-driven responses in human mast cells. Journal of Immunotoxicology, 14 (1). pp. 60-65. ISSN 1547-691X
Abstract
As part of the intracellular processes leading to mast cell and basophil activation, phosphorylation of key substrates is likely to be important. These processes, mediated by phosphatases, are responsible for regulating phosphorylation. The aim of the present study was to determine effects fostriecin – a selective inhibitor of PP2A (protein phosphatase-2) – on β2-adrenoceptor-driven responses in human mast cells. Here, the effects of fostriecin (PP inhibitors) on the inhibition of histamine release from HLMC, on β-adrenoceptor-driven responses in mast cells and on desensitization were investigated. Long-term incubation (24 h) of mast cells with fostriecin (10−6 M) resulted in a significant (p < 0.001) reduction in the maximal response (from 41.2 [± 3.0] to 29.9 [± 4.2] %) to salbutamol following fostriecin treatment. The results showed that fostriecin pretreatment significantly attenuated the inhibitory effects of salbutamol. Overall, the present study suggested that PP2A has an important role in regulating mast cell β2-adrenoceptors.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Desensitization; histamine; mast cell; fostriecin |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 15 Mar 2018 12:57 |
Last Modified: | 15 Mar 2018 12:57 |
Published Version: | https://doi.org/10.1080/1547691X.2016.1259277 |
Status: | Published |
Publisher: | Informa Healthcare |
Refereed: | Yes |
Identification Number: | 10.1080/1547691X.2016.1259277 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128546 |
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