Lermyte, F, Valkenborg, D, Loo, JA et al. (1 more author) (2018) Radical solutions: Principles and application of electron‐based dissociation in mass spectrometry‐based analysis of protein structure. Mass Spectrometry Reviews, 37 (6). pp. 750-771. ISSN 0277-7037
Abstract
In recent years, electron capture (ECD) and electron transfer dissociation (ETD) have emerged as two of the most useful methods in mass spectrometry‐based protein analysis, evidenced by a considerable and growing body of literature. In large part, the interest in these methods is due to their ability to induce backbone fragmentation with very little disruption of noncovalent interactions which allows inference of information regarding higher order structure from the observed fragmentation behavior. Here, we review the evolution of electron‐based dissociation methods, and pay particular attention to their application in “native” mass spectrometry, their mechanism, determinants of fragmentation behavior, and recent developments in available instrumentation. Although we focus on the two most widely used methods—ECD and ETD—we also discuss the use of other ion/electron, ion/ion, and ion/neutral fragmentation methods, useful for interrogation of a range of classes of biomolecules in positive‐ and negative‐ion mode, and speculate about how this exciting field might evolve in the coming years.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2018, Wiley Periodicals, Inc. This is the peer reviewed version of the following article: 'Lermyte, F, Valkenborg, D, Loo, JA, and Sobott, F (2018). Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure. Mass Spectrometry Reviews,' which has been published in final form at [https://doi.org/10.1002/mas.21560]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Keywords: | electron capture dissociation; electron transfer dissociation; noncovalent complex; protein folding; top-down fragmentation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Mar 2018 14:59 |
Last Modified: | 09 Feb 2019 01:38 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/mas.21560 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128023 |