Thompson, Andrew J. orcid.org/0000-0001-7865-1856, Spears, Richard J., Zhu, Yanping et al. (4 more authors) (2018) Bacteroides thetaiotaomicron generates diverse α-mannosidase activities through subtle evolution of a distal substrate-binding motif. Acta crystallographica. Section D, Structural biology. pp. 394-404. ISSN 2059-7983
Abstract
A dominant human gut microbe, the well studied symbiont Bacteroides thetaiotaomicron (Bt), is a glyco-specialist that harbors a large repertoire of genes devoted to carbohydrate processing. Despite strong similarities among them, many of the encoded enzymes have evolved distinct substrate specificities, and through the clustering of cognate genes within operons termed polysaccharide-utilization loci (PULs) enable the fulfilment of complex biological roles. Structural analyses of two glycoside hydrolase family 92 α-mannosidases, BT3130 and BT3965, together with mechanistically relevant complexes at 1.8–2.5 Å resolution reveal conservation of the global enzyme fold and core catalytic apparatus despite different linkage specificities. Structure comparison shows that Bt differentiates the activity of these enzymes through evolution of a highly variable substrate-binding region immediately adjacent to the active site. These observations unveil a genetic/biochemical mechanism through which polysaccharide-processing bacteria can evolve new and specific biochemical activities from otherwise highly similar gene products.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | 2018, The Author(s). |
Keywords: | Bacteroides thetaiotaomicron,carbohydrates,glycans,glycoside hydrolase family 92,substrate specificity,α-mannosidase |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Arts and Humanities (York) > History (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/G016127/1 |
Depositing User: | Pure (York) |
Date Deposited: | 21 Feb 2018 10:10 |
Last Modified: | 17 Dec 2024 00:09 |
Published Version: | https://doi.org/10.1107/S2059798318002942 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1107/S2059798318002942 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:127792 |