Presslee, Samantha Louise, Wilson, Julie Carol orcid.org/0000-0002-5171-8480, Collins, Matthew James orcid.org/0000-0003-4226-5501 et al. (1 more author) (2018) The identification of archaeological eggshell using peptide markers. STAR: Science Technology of Archaeological Research. 4:1. pp. 13-23. ISSN 2054-8923
Abstract
Avian eggshell survives well in alkaline and neutral soils, but its potential as an archaeological resource remains largely unexplored, largely due to difficulties in its identification. Here we exploit the release of novel bird genomes and, for the first time on eggshell, use MALDI-ToF mass spectrometry in combination with peptide sequencing by LC-MS/MS. The eggshell proteome is revealed as unexpectedly complex, with 5755 proteins identified for a reference collection comprising 23 bird species. We determined 782 m/z markers useful for eggshell identification, 583 of which could be assigned to known eggshell peptide sequences. These were used to identify eggshell fragments recovered from a medieval site at Freeschool Lane, Leicester. We discuss the specificity of the peptide markers and highlight the importance of assessing the level of taxonomic identification achievable for archaeological interpretation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Arts and Humanities (York) > Archaeology (York) The University of York > Faculty of Sciences (York) > Mathematics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 31 Jan 2018 16:50 |
Last Modified: | 23 Jan 2025 00:14 |
Published Version: | https://doi.org/10.1080/20548923.2018.1424300 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1080/20548923.2018.1424300 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:126895 |
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Description: The identification of archaeological eggshell using peptide markers
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