Wilkinson, R.N., Elworthy, S., Ingham, P.W. et al. (1 more author) (2017) Fin clipping and genotyping embryonic zebrafish at 3 days post-fertilization. BioTechniques, 62 (1). xxiv. ISSN 0736-6205
Abstract
Protocol Summary
Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.
Protocol Schematic
Embryonic fin transection (A) Dotted line represents the site of transection at three days post-fertilization (dpf), black arrowhead highlights the pigment gap; white arrowhead, highlights the caudal limit of blood circulation. (B) Untransected embryonic fin at five dpf. (C) Embryonic zebrafish fin at five dpf, two days post-transection. Black arrowhead highlights blastema formation and ongoing fin regeneration.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 BioTechniques. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Funding Information: | Funder Grant number ROYAL SOCIETY RG120564 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 31 Jan 2018 10:45 |
Last Modified: | 31 Jan 2018 10:45 |
Published Version: | https://doi.org/10.2144/000114509 |
Status: | Published |
Publisher: | Informa BioSciences |
Refereed: | Yes |
Identification Number: | 10.2144/000114509 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:126853 |