Di Silvio, D, Rigby, N, Bajka, B et al. (2 more authors) (2016) Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake. International Journal of Biochemistry and Cell Biology, 75. pp. 212-222. ISSN 1357-2725
Abstract
Nanoparticles (NPs) in biological fluids immediately interact with proteins forming a biomolecular corona (PC) that imparts their biological identity. While several studies on the formation of the PC in human plasma have been reported, the PC of orally administrated NPs has been less investigated, mostly in the presence of a food matrix. In fact, food matrixes when digested are subject of several dynamic changes that will certainly affect the PC formed on the NPs. The lack of studies on this topic is clearly related to the difficulty in isolating representative PC NPs from such a complex environment. In this work magnetite NPs were added to in vitro simulated digestion simultaneously with bread and PC NPs were isolated after gastric and duodenal phases by sucrose gradient ultracentrifugation (UC). The PC NPs were characterized in terms of size and protein composition. Translocation studies were then performed on Caco-2 monolayers in a serum free environment and cell morphology was characterized by confocal microscopy. PC NPs isolated from gastric and duodenal phases were different in size, surface charge and protein corona composition. NP cellular uptake was enhanced by the digestive PC inducing morphology changes in the cell monolayer. Overall, in this work we were able to isolate PC NPs from digested fluids in the presence of a food matrix and study their biological response on Caco-2 cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Protein corona; Nanoparticle; Gastrointestinal digestion; Bread; Caco-2; Cell uptake |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Feb 2018 14:29 |
Last Modified: | 19 Feb 2018 14:29 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.biocel.2015.10.019 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:126697 |