Thorne, JL orcid.org/0000-0002-3037-8528, Ouboussad, L and Lefevre, PF (2012) Heterochromatin protein 1 gamma and IiB kinase alpha interdependence during tumour necrosis factor gene transcription elongation in activated macrophages. Nucleic Acids Research, 40 (16). pp. 7676-7689. ISSN 0305-1048
Abstract
IiB kinase a (IKKa) is part of the cytoplasmic IKK complex regulating nuclear factor-{kappa}B (NF-iB) release and translocation into the nucleus in response to pro-inflammatory signals. IKKa can also be recruited directly to the promoter of NF-iB-dependent genes by NF-iB where it phosphorylates histone H3 at serine 10, triggering recruitment of the bromodomain-containing protein 4 and the positive transcription elongation factor b. Herein, we report that IKKa travels with the elongating form of ribonucleic acid polymerase II together with heterochromatin protein 1 gamma (HP1c) at NF-iBdependent genes in activated macrophages. IKKa binds to and phosphorylates HP1c, which in turn controls IKKa binding to chromatin and phosphorylation of the histone variant H3.3 at serine 31 within transcribing regions. Downstream of transcription end sites, IKKa accumulates with its inhibitor the CUE-domain containing protein 2, suggesting a link between IKKa inactivation and transcription termination.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2012, The Author(s). Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Experimental Haematology (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Clinical Musculoskeletal Medicine (LIRMM) (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 09 Sep 2019 14:37 |
Last Modified: | 09 Sep 2019 14:37 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1093/nar/gks509 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:126439 |