Purdie, L., Alexander, C., Spain, S.G. orcid.org/0000-0001-7241-5713 et al. (1 more author) (2018) Alkyl-modified oligonucleotides as intercalating vehicles for doxorubicin uptake via albumin binding. Molecular Pharmaceutics, 15 (2). pp. 437-446. ISSN 1543-8384
Abstract
DNA-based drug delivery vehicles have displayed promise for the delivery of intercalating drugs. Here, we demonstrate that oligonucleotides modified with an alkyl chain can bind to human serum albumin, mimicking the natural binding of fatty acids. These alkyl-DNA–albumin complexes display excellent serum stability and are capable of strongly binding doxorubicin. Complexes are internalized by cells in vitro, trafficking to the mitochondria, and are capable of delivering doxorubicin with excellent efficiency resulting in cell death. However, the cellular localization of the delivered doxorubicin, and ultimately the complex efficacy, is dependent on the nature of the linker between the alkyl group and the oligonucleotide.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 American Chemical Society. |
Keywords: | Alkyls; Antineoplastic agents; Biopolymers; Conjugate acid-base pairs; Peptides and proteins |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 22 Dec 2017 10:40 |
Last Modified: | 23 Feb 2024 15:17 |
Status: | Published |
Publisher: | American Chemical Society |
Refereed: | Yes |
Identification Number: | 10.1021/acs.molpharmaceut.7b00805 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:125505 |