Actis, P orcid.org/0000-0002-7146-1854 (2018) Sampling from single cells. Small Methods, 2 (3). 1700300.
Abstract
The cell is the fundamental unit of biology. Major methodological advances in engineering and molecular biology have enabled the 'omics analysis of individual cells and supported biologists in understanding the deepest difference between health and disease. These advancements were based on the assumption that a single cell needs to be lysed or fixed before any in‐depth analysis can be performed. Here, a picture is painted of the innovative methods used for extracting the contents of living cells without affecting their viability. These novel methods are now empowering the biological community to repeatedly interrogate a single cell over time, thus giving a dynamic representation of the cell's 'omics rather than a snapshot at a particular time point.
Metadata
Item Type: | Article |
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Authors/Creators: | |
Copyright, Publisher and Additional Information: | © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Actis, P (2018) Sampling from single cells. Small Methods, 2 (3), which has been published in final form at https://doi.org/10.1002/smtd.201700300. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Keywords: | atomic force microscopy (AFM); nanobiopsy; nanopipettes; scanning ion conductance microscopy (SICM); single-cell genomics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 20 Nov 2017 13:06 |
Last Modified: | 26 Jan 2019 01:38 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/smtd.201700300 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:124246 |