Meshcheriakova, Y, Durrant, A, Hesketh, EL et al. (2 more authors) (2017) Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. Biochemical Society Transactions, 45 (6). pp. 1263-1269. ISSN 0300-5127
Abstract
Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of possible applications for CPMV-based particles. However, for this potential to be realised, it was essential to demonstrate that eVLPs were accurate surrogates for natural virus particles, and this information was provided by high-resolution cryo-EM studies of eVLPs. This demonstration has enabled the approaches developed for the production of modified particles developed with natural CPMV particles to be applied to eVLPs. Furthermore, a combination of cryo-EM and mutagenic studies allowed the development of particles which are permeable but which could still assemble efficiently. These particles were shown to be loadable with cobalt, indicating that they can, indeed, be used as nano-containers.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2017 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). (https://creativecommons.org/licenses/by/4.0/) |
Keywords: | cowpea mosaic virus; cryo-electron microscopy; mutagenesis; transient expression; virus-like particle |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cryo EM, Image Processing (Leeds) |
Funding Information: | Funder Grant number BBSRC BB/L021250/1 BBSRC BB/C511372/1 Wellcome Trust 094232/Z/10/Z Wellcome Trust 094232/Z/10/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 15 Nov 2017 11:26 |
Last Modified: | 23 Jun 2023 22:39 |
Status: | Published |
Publisher: | Portland Press |
Identification Number: | 10.1042/BST20160312 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:124082 |