Kyriakides, O., Halliwell, J.A. and Andrews, P.W. orcid.org/0000-0001-7215-4410 (2018) Acquired genetic and epigenetic variation in human pluripotent stem cells. Advances in Biochemical Engineering Biotechnology, 163. pp. 187-206. ISSN 0724-6145
Abstract
Human pluripotent stem cells (hPSCs) can acquire non-random genomic variation during culture. Some of these changes are common in tumours and confer a selective growth advantage in culture. Additionally, there is evidence that reprogramming of human induced pluripotent stem cells (hiPSCs) introduces mutations. This poses a challenge to both the safety of clinical applications and the reliability of basic research using hPSCs carrying genomic variation. A number of methods are available for monitoring the genomic integrity of hPSCs, and a balance between practicality and sensitivity must be considered in choosing the appropriate methods for each use of hPSCs. Adjusting protocols by which hPSCs are derived and cultured is an evolving process that is important in minimising acquired genomic variation. Assessing genetic variation for its potential impact is becoming increasingly important as techniques to detect genome-wide variation improve.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Springer International Publishing AG. This is an author produced version of a paper subsequently published in Advances in Biochemical Engineering Biotechnology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Cytogenetics; Epigenetic; Genetic variants; Human; Karyotype; Pluripotent stem cells |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 23 Nov 2017 10:35 |
Last Modified: | 14 Dec 2023 15:02 |
Status: | Published |
Publisher: | Springer Verlag |
Refereed: | Yes |
Identification Number: | 10.1007/10_2017_22 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123687 |