Ilett, M, Bamiduro, F orcid.org/0000-0002-1914-5731, Matar, O et al. (3 more authors) (2017) Cryo-STEM-EDX spectroscopy for the characterisation of nanoparticles in cell culture media. In: Journal of Physics: Conference Series. Electron Microscopy and Analysis Group Conference 2017 (EMAG2017), 03-06 Jul 2017, Manchester, UK. IOP Publishing
Abstract
We present a study of barium titanate nanoparticles dispersed in cell culture media. Scanning transmission electron microscopy combined with energy dispersive X-ray spectroscopy was undertaken on samples prepared using both conventional drop casting and also plunge freezing and examination under cryogenic conditions. This showed that drying artefacts occurred during conventional sample preparation, whereby some salt components of the cell culture media accumulated around the barium titanate nanoparticles; these were removed using the cryogenic route. Importantly, the formation of a calcium and phosphorus rich coating around the barium titanate nanoparticles was retained under cryo-conditions, highlighting that significant interactions do occur between nanomaterials and biological media.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017. Published under licence by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) |
Funding Information: | Funder Grant number EPSRC EP/M028143/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Nov 2017 15:49 |
Last Modified: | 08 Nov 2017 08:42 |
Status: | Published |
Publisher: | IOP Publishing |
Identification Number: | 10.1088/1742-6596/902/1/012006 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123579 |