Byers, R.A., Fisher, M., Brown, N.J. orcid.org/0000-0002-2100-1905 et al. (2 more authors) (2017) Vascular patterning of subcutaneous mouse fibrosarcomas expressing individual VEGF isoforms can be differentiated using angiographic optical coherence tomography. Biomedical Optics Express, 8 (10). pp. 4551-4567. ISSN 2156-7085
Abstract
Subcutaneously implanted experimental tumors in mice are commonly used in cancer research. Despite their superficial location, they remain a challenge to image noninvasively at sufficient spatial resolution for microvascular studies. Here we evaluate the capabilities of optical coherence tomography (OCT) angiography for imaging such tumors directly through the murine skin in-vivo. Datasets were collected from mouse tumors derived from fibrosarcoma cells genetically engineered to express only single splice variant isoforms of vascular endothelial growth factor A (VEGF); either VEGF120 or VEGF188 (fs120 and fs188 tumors respectively). Measured vessel diameter was found to be significantly (p<0.001) higher for fs120 tumors (60.7±4.9μm) compared to fs188 tumors (45.0±4.0μm). The fs120 tumors also displayed significantly higher vessel tortuosity, fractal dimension and density. The ability to differentiate between tumor types with OCT suggests that the visible abnormal vasculature is representative of the tumor microcirculation, providing a robust, non-invasive method for observing the longitudinal dynamics of the subcutaneous tumor microcirculation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Optical Society of America. This is an author produced version of a paper subsequently published in Biomedical Optics Express. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Electronic and Electrical Engineering (Sheffield) The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 03 Nov 2017 11:46 |
Last Modified: | 28 Mar 2018 19:28 |
Published Version: | https://doi.org/10.1364/BOE.8.004551 |
Status: | Published |
Publisher: | Optical Society of America |
Refereed: | Yes |
Identification Number: | 10.1364/BOE.8.004551 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123369 |