Kanatas, A orcid.org/0000-0003-2025-748X, Chengot, P, Ong, TK et al. (5 more authors) (2018) Genomic analysis to assess disease progression and recurrence in patients with oral squamous cell carcinoma: – a preliminary study. British Journal of Oral and Maxillofacial Surgery, 56 (3). pp. 198-205. ISSN 0266-4356
Abstract
We studied the progression from dysplasia to invasive carcinoma and subsequent second primaries or locoregional recurrences in 11 patients with recurrent squamous cell carcinoma (SCC). Between one and six samples were sequenced/patient. DNA samples were prepared, and libraries multiplexed to between 40 and 80 samples/lane of an Illumina HiSeq 3000 and sequenced with 2 × 100 bp paired end sequencing. Copy number data were generated by CNAnorm (Bioconductor package). Samples of recurrent SCC showed unique patterns of descent when compared with earlier samples from the primary tumour, and three main patterns emerged. In four patients there was convincing evidence that the later lesion was descended directly from cells from the first, and in a further four there were no detectable genomic events between the two lesions. Three patients had some shared events between the early and later lesions, but although there were enough differences to deduce that the two lesions had a shared ancestor, they were not directly descended from each other. We present the patients’ characteristics in detail, including the overall survival in each group. There was a distinct genomic pattern after a second episode of SCC in all the groups. A larger study that uses similar methods and a longer duration could provide reliable conclusions with respect to survival. With the use of new techniques, genomic data can be available to clinical teams during the planning of treatment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. This is an author produced version of a paper published in British Journal of Oral and Maxillofacial Surgery. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Oral cancer; Squamous cell carcinoma; Recurrence; Oral dysplasia; DNA sequencing; Genomics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > School of Dentistry Visitors (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cancer and Pathology (LICAP) > Pathology & Tumour Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 Nov 2017 11:14 |
Last Modified: | 01 Feb 2019 01:47 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bjoms.2018.01.010 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123359 |