Tassi, M, De Vos, J, Chatterjee, S et al. (3 more authors) (2018) Advances in native high-performance liquid chromatography and intact mass spectrometry for the characterization of biopharmaceutical products. Journal of Separation Science, 41 (1). pp. 125-144. ISSN 1615-9306
Abstract
The characterization of biotherapeutics represents a major analytical challenge. This review discusses the current state-of-the-art in analytical technologies to profile biopharma products under native conditions, i.e., the protein 3D conformation is maintained during liquid chromatographic analysis. Native liquid-chromatographic modes that are discussed include aqueous size-exclusion chromatography, hydrophobic interaction chromatography, and ion-exchange chromatography. Infusion conditions and the possibilities and limitations to hyphenate native liquid chromatography to mass spectrometry are discussed. Furthermore, the applicability of native liquid-chromatography methods and intact mass-spectrometry analysis for the characterization of monoclonal antibodies and antibody-drug conjugates is discussed.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: 'Tassi, M., De Vos, J., Chatterjee, S et al. Advances in native high-performance liquid chromatography and intact mass spectrometry for the characterization of biopharmaceutical products. Journal of Separation Science, 41 (1). pp. 125-144.' Published in final form at https://doi.org/10.1002/jssc.201700988. This article may be used for non-commercial purposes in accordance with the Wiley Terms and Conditions for Self-Archiving. |
Keywords: | protein therapeutics, bioprocessing, native chromatography methods, intact and native mass spectrometry, ADC, mAb. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 Nov 2017 13:14 |
Last Modified: | 09 Oct 2018 00:39 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/jssc.201700988 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123356 |