Ali, F., Sreedharan, S., Ashoka, A.H. et al. (4 more authors) (2017) A super resolution probe to monitor HNO levels in the endoplasmic reticulum of cells. Analytical Chemistry , 89 (22). pp. 12087-12093. ISSN 0003-2700
Abstract
Selective detection of nitroxyl (HNO), which has recently been identified as a reactive nitrogen species, is a challenging task. We report a BODIPY-based luminescence ON reagent for detection of HNO in aqueous solution and in live RAW 264.7 cells, based on the soft nucleophilicity of the phosphine oxide functionality towards HNO. The probe shows high selectivity to HNO over other reactive oxygen/nitrogen and sulphur species. Luminescence properties of the BODIPY based chemodosimetric reagent make it an ideal candidate for use as a reagent for super resolution structured illumination microscopy. The viability of the reagent for biological in-vivo imaging application was also confirmed using Artemia as a model.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.analchem.7b02567. |
Keywords: | HNO; luminescence; superresolution imaging; endoplasmic reticulum |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 31 Oct 2017 13:27 |
Last Modified: | 14 Dec 2023 11:12 |
Status: | Published |
Publisher: | American Chemical Society |
Refereed: | Yes |
Identification Number: | 10.1021/acs.analchem.7b02567 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123245 |