Wang, Y, Chen, S, Ni, Y et al. (7 more authors) (2017) Three-dimensional reconstruction with serial whole-mount sections of oral tongue squamous cell carcinoma: a preliminary study. Journal of Oral Pathology and Medicine. ISSN 0904-2512
Abstract
Objectives: Margin status and invasion pattern are prognostic factors for oral tongue squamous cell carcinoma (OTSCC). Current methods to identify these factors are limited to 2D observation; it is necessary to explore 3D reconstruction with whole-mount sample to improve the accuracy of analysis. This study aimed to study the tissue preparation, section generation, and 3D reconstruction with whole-mount OTSCC specimen. Study design: Two OTSCC samples were retrieved from Nanjing Stomatological Hospital, Medical School of Nanjing University. One sample was sliced into 3 equal-sized pieces and subjected to different processing schedules to determine the best method. The second sample was processed accordingly. Serial whole-mount sections of the second sample were generated, stained with HE/anticytokine antibody in intersection manner, and scanned into digital images. Digital images were aligned and reconstructed into 3D images with Hetero Genius Medical Image Manager 3D Pathology Add-On [HGMIM3D]. Results: Successful serial whole-mount sections of comparable quality to traditional sections were generated. Three-dimensional images with serial whole-mount sections were successfully generated. Conclusions: Whole-mount histopathological 3D reconstruction of OTSCC was successfully generated, providing a solid foundation for comprehensive margin and invasion analysis. Although future study and improvement were needed, whole-mount histopathological 3D reconstruction proved to be a promising method in OTSCC study.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Wang Y, Chen S, Ni Y, et al. Three-dimensional reconstruction with serial whole-mount sections of oral tongue squamous cell carcinoma: A preliminary study. J Oral Pathol Med. 2017;00:1–7. https://doi.org/10.1111/jop.12644, which has been published in final form at https://doi.org/10.1111/jop.12644. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | invasion; margin status; oral tongue squamous cell carcinoma; serial whole-mount sections; three-dimensional reconstruction |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Computing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Oct 2017 12:07 |
Last Modified: | 28 Sep 2018 00:39 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/jop.12644 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:122981 |