Uddin, SZ and Talukder, MA orcid.org/0000-0002-2814-3658 (2017) Reduction of detection volume in total internal reflection fluorescence microscopy using graphene. In: Proceedings of 9th International Conference on Electrical and Computer Engineering (ICECE 2016). ICECE 2016, 20-22 Dec 2016, Dhaka, Bangladesh. IEEE , pp. 143-146. ISBN 9781509029631
Abstract
We show that it is possible to decrease the thickness of the detection volume of total internal reflection fluorescence microscopy (TIRFM) by ~35% using a graphene layer in the interface between glass and water layers of a typical TIRFM structure without sacrificing the fluorescence intensity. The highly mobile surface bound electrons of a graphene mono-layer quenches the fluorophores that are less than ~40 nm away from it. The decreased detection volume using the proposed structure will increase the resolution of' a typical TIRFM technique. We find that the results are qualitatively similar for different incidence angles and polarizations of' the excitation field. So, the proposed structure will also find applications in variants of TIRFM techniques, e.g., where incidence angles and polarizations are varied.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2016, IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. |
Keywords: | Detection Volume, Total Internal Reflection Fluorescence Microscopy, Graphene; Fluorescence, Reflection, Glass, Chemicals, Permittivity |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Sep 2017 15:15 |
Last Modified: | 15 Jan 2018 20:57 |
Status: | Published |
Publisher: | IEEE |
Identification Number: | 10.1109/ICECE.2016.7853876 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:121207 |