Som, NF, Heine, D, Holmes, N et al. (6 more authors) (2017) The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2. Microbiology, 163 (10). pp. 1415-1419. ISSN 1350-0872
Abstract
MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezuelae. Chloramphenicol biosynthesis is directly regulated by MtrA in S. venezuelae and deletion of mtrB constitutively activates MtrA and results in constitutive over-production of chloramphenicol. Here we report that in Streptomyces coelicolor, MtrA binds to sites upstream of developmental genes and the genes encoding ActII-1, ActII-4 and RedZ, which are cluster-situated regulators of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red). Consistent with this, deletion of mtrB switches on the production of Act, Red and streptorubin B, a product of the Red pathway. Thus, we propose that MtrA is a key regulator that links antibiotic production to development and can be used to upregulate antibiotic production in distantly related streptomycetes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2017 The Authors, This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. |
Keywords: | porulation, Streptomyces, cryptic gene clusters, antibiotics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 31 Aug 2017 09:54 |
Last Modified: | 08 Apr 2018 13:47 |
Status: | Published |
Publisher: | Microbiology Society |
Identification Number: | 10.1099/mic.0.000524 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:120678 |
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Licence: CC-BY 4.0
Filename: 000524.pdf
Filename: Copy of Table S2 - Complete S coelicolor dataset.xls
Description: Dataset