Rosenzweig, Elizabeth S Freeman, Xu, Bin, Cuellar, Luis Kuhn et al. (11 more authors) (2017) The eukaryotic CO2-concentrating organelle is liquid-like and exhibits dynamic reorganization. Cell. 148-162.e19. ISSN 1097-4172
Abstract
Approximately 30%–40% of global CO 2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO 2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a “magic number” effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | Copyright © 2017 Elsevier Inc. All rights reserved. |
Keywords: | CO concentrating mechanism,Chlamydomonas reinhardtii,Rubisco,biological phase transitions,carbon fixation,cryo-electron tomography,liquid-like organelles,magic numbers,organelle inheritance,pyrenoid,Chloroplasts/chemistry,Cryoelectron Microscopy,Organelle Biogenesis,Carbon Dioxide/metabolism,Ribulose-Bisphosphate Carboxylase/metabolism,Chlamydomonas reinhardtii/chemistry,Algal Proteins/metabolism |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 30 Aug 2017 08:00 |
Last Modified: | 16 Oct 2024 14:00 |
Published Version: | https://doi.org/10.1016/j.cell.2017.08.008 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.cell.2017.08.008 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:120656 |
Downloads
Filename: 170716_Freeman_Rosenzweig_et_al.pdf
Description: 170716_Freeman_Rosenzweig_et_al
Filename: Freeman_Rosenzweig_Cell_AAM.zip
Description: Supplementary files