Mackie, Meaghan, Hendy, Jessica Ruth orcid.org/0000-0002-3718-1058, Lowe, Abigail Daisy et al. (4 more authors) (2017) Preservation of the metaproteome::Variability of protein preservation in ancient dental calculus. STAR: Science Technology of Archaeological Research. pp. 1-14.
Abstract
Proteomic analysis of dental calculus is emerging as a powerful tool for disease and dietary characterisation of archaeological populations. To better understand the variability in protein results from dental calculus, we analysed 21 samples from three Roman-period populations to compare: 1) the quantity of extracted protein; 2) the number of mass spectral queries; and 3) the number of peptide spectral matches and protein identifications. We found little correlation between the quantity of calculus analysed and total protein identifications, as well as no systematic trends between site location and protein preservation. We identified a wide range of individual variability, which may be associated with the mechanisms of calculus formation and/or post-depositional contamination, in addition to taphonomic factors. Our results suggest dental calculus is indeed a stable, long-term reservoir of proteins as previously reported, but further systematic studies are needed to identify mechanisms associated with protein entrapment and survival in dental calculus.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. |
Keywords: | Archaeology,dental calculus,destructive analysis,LC-MS/MS,Shotgun proteomics,protein preservation |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Arts and Humanities (York) > Archaeology (York) |
Funding Information: | Funder Grant number UNSPECIFIED 108375/Z/15/Z |
Depositing User: | Pure (York) |
Date Deposited: | 14 Aug 2017 09:00 |
Last Modified: | 13 Nov 2024 09:08 |
Published Version: | https://doi.org/10.1080/20548923.2017.1361629 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1080/20548923.2017.1361629 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:120116 |