Borodavka, A orcid.org/0000-0002-5729-2687, Dykeman, EC, Schrimpf, W et al. (1 more author) (2017) Protein-mediated RNA folding governs sequence-specific interactions between rotavirus genome segments. eLife, 6. e27453. ISSN 2050-084X
Abstract
Segmented RNA viruses are ubiquitous pathogens, which include influenza viruses and rotaviruses. A major challenge in understanding their assembly is the combinatorial problem of a non-random selection of a full genomic set of distinct RNAs. This process involves complex protein/RNA interactions, which are often obscured by non-specific binding at concentrations approaching in vvo assembly conditions. Here, we present direct experimental evidence of sequence-specific inter-segment interactions in rotaviruses, taking place in a complex RNA- and protein- rich milieu. We show that binding of the rotaviral protein NSP2 to ssRNAs results in the remodeling of RNA, which is conducive to formation of inter-segment contacts. To identify the sites of these interactions, we have developed an RNA-RNA SELEX approach for mapping the sequences involved in inter-segment base-pairing. Our findings elucidate the molecular basis underlying inter-segment interactions in rotaviruses, paving the way for delineating RNA-RNA interactions that govern assembly of other segmented RNA viruses
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Borodavka et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
Keywords: | Biophysics and Structural Biology; Microbiology and Infectious Disease; Rotavirus; RNA-RNA interactions; RNA folding; Virus assembly; Fluorescence Cross-Correlation Spectroscopy |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Bioinformatics (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 103068/Z/13/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Aug 2017 10:14 |
Last Modified: | 17 Jan 2018 04:37 |
Status: | Published |
Publisher: | eLife Sciences Publications |
Identification Number: | 10.7554/eLife.27453.001 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:119836 |