Roedig, P, Ginn, HM, Pakendorf, T et al. (26 more authors) (2017) High-speed fixed-target serial virus crystallography. Nature Methods, 14 (8). pp. 753-825. ISSN 1548-7091
Abstract
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2017, Author(s). This is an author produced version of a paper published in Nature Methods. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Nanocrystallography; Proteins; Virology |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 03 Jul 2017 12:14 |
Last Modified: | 19 Dec 2017 01:39 |
Status: | Published |
Publisher: | Nature Publishing Group |
Identification Number: | 10.1038/nmeth.4335 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:118468 |