Plant, N (2015) Molecular biology II: protein function. Surgery (Oxford), 33 (3). pp. 99-103. ISSN 0263-9319
Abstract
DNA may be viewed as the blueprint for the body, with mRNA/protein the component parts made from this blueprint. However, it is only when proteins are allowed to interact with each other and their surroundings that true biological complexity is achieved. Thus, while it is informative to study transcriptional control and mRNA transcript levels, it is equally important to assess the impact of the encoded proteins on the total cellular environment. For example, expression of a ligand-activated receptor such as the epidermal growth factor (EGF) receptor is of no biological consequence if no EGF is present in the system. It is thus important to be able to study protein interactions and modification, enabling a comprehensive understanding of the biological mechanisms that underlie any particular phenotype. This article will outline the basic technologies to both visualize protein localization and interaction between co-localized proteins. In addition, the manipulation of protein levels, both in vitro and in vivo, will be described, as this provides an important tool for the further examination of protein functionality within biological systems
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2017, Elsevier Ltd. All rights reserved. This is an author produced version of a paper published in Surgery (Oxford). Uploaded in accordance with the publisher's self-archiving policy |
Keywords: | Co-localization; ES cells; FRET; GFP; RNAi; transgenic animals |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Sep 2017 14:10 |
Last Modified: | 30 Jan 2018 20:17 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.mpsur.2015.01.002 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:118399 |