Mulakhudair, A.R., Al-Mashhadani, M., Hanotu, J. et al. (1 more author) (2017) Inactivation combined with cell lysis of Pseudomonas putida using a low pressure carbon dioxide microbubble technology. Journal of Chemical Technology & Biotechnology, 92 (8). pp. 1961-1969. ISSN 0268-2575
Abstract
BACKGROUND Inactivation processes can be classified into non-thermal inactivation methods such as ethylene oxide and γ-radiation, and thermal methods such as autoclaving. The ability of carbon dioxide enriched microbubbles to inactivate Pseudomonas putida suspended in physiological saline, as a non-thermal sterilisation method, was investigated in this study with many operational advantages over both traditional thermal and non-thermal sterilisation methods.
RESULTS Introducing carbon dioxide enriched microbubbles can achieve ∼2-Log reduction in the bacterial population after 90 min of treatment, addition of ethanol to the inactivation solution further enhanced the inactivation process to achieve 3, 2.5 and 3.5-Log reduction for 2%, 5% and 10 %( v/v) ethanol, respectively. A range of morphological changes was observed on Pseudomonas cells after each treatment, and these changes extended from changing cell shape from rod shape to coccus shape to severe lesions and cell death. Pseudomonas putida KT 2440 was used as a model of gram-negative bacteria.
CONCLUSION Using CO2 enriched microbubbles technology has many advantages such as efficient energy consumption (no heat source), avoidance of toxic and corrosive reagents, and in situ treatment. In addition, many findings from this study could apply to other gram-negative bacteria.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | inactivation;cell lysis;CO2;microbubbles;Pseudomonas |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/N011511/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 03 Jul 2017 08:37 |
Last Modified: | 03 Jul 2017 08:37 |
Published Version: | https://doi.org/10.1002/jctb.5299 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/jctb.5299 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:118014 |
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