Wood, Ian P, Pearson, Bruce M, Garcia-Gutierrez, Enriqueta et al. (5 more authors) (2017) Carbohydrate microarrays and their use for the identification of molecular markers for plant cell wall composition. Proceedings of the National Academy of Sciences of the United States of America. pp. 6860-6865. ISSN 1091-6490
Abstract
Genetic improvement of the plant cell wall has enormous potential to increase the quality of food, fibers, and fuels. However, the identification and characterization of genes involved in plant cell wall synthesis is far from complete. Association mapping is one of the few techniques that can help identify candidate genes without relying on our currently incomplete knowledge of cell wall synthesis. However, few cellwall phenotypingmethodologies have proven sufficiently precise, robust, or scalable for association mapping to be conducted for specific cell wall polymers. Here, we created highdensity carbohydrate microarrays containing chemically extracted cell wall polysaccharides collected from 331 genetically diverse Brassica napus cultivars and used them to obtain detailed, quantitative information describing the relative abundance of selected noncellulosic polysaccharide linkages and primary structures. We undertook genome-wide association analysis of data collected from 57 carbohydrate microarrays and identified molecular markers reflecting a diversity of specific xylan, xyloglucan, pectin, and arabinogalactan moieties. These datasets provide a detailed insight into the natural variations in cell wall carbohydrate moieties between B. napus genotypes and identify associated markers that could be exploited by marker-assisted breeding. The identified markers also have value beyond B. napus for functional genomics, facilitated by the close genetic relatedness to the model plant Arabidopsis. Together, our findings provide a unique dissection of the genetic architecture that underpins plant cell wall biosynthesis and restructuring.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details |
Keywords: | Association mapping,Biomass,Functional genomics,GWAS,Lignocellulose,Carbohydrates,Species Specificity,Microarray Analysis,Cell Wall/metabolism,Brassica napus/metabolism,Carbohydrate Metabolism/physiology,Databases, Factual |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) > Centre for Novel Agricultural Products (CNAP) (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 16 Jun 2017 09:30 |
Last Modified: | 21 Jan 2025 17:27 |
Published Version: | https://doi.org/10.1073/pnas.1619033114 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1073/pnas.1619033114 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117852 |
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