Adeyemi, OO orcid.org/0000-0002-0848-5917, Herod, MR, Oladiji, F et al. (3 more authors) (2017) A multi-template multiplex PCR assay for hepatitis B virus and human β-globin. Journal of Medical Virology, 89 (11). pp. 1944-1951. ISSN 0146-6615
Abstract
The Hepatitis B surface antigen (HBsAg) is the hallmark of HBV infection. Detection of antibodies to HBs and the core (i.e. HBsAg and HBcAb) are primary serological algorithms in the laboratory diagnosis of HBV. Detection of HBsAg DNA is an important supplement to serological diagnosis especially in clinical cases. Simultaneous amplification of internal cellular controls is a good indicator of sample quality. Human P-globin is a well characterised housekeeping gene (HKG) that is often applied as internal controls (IC) in molecular diagnosis. In this study, individual plasmid clones of the human P-globin and HBs genes were constructed. These plasmid constructs have been applied to characterise a multiplex PCR assays for HBs and P-globin genes. The findings suggest detection limits of less than 10 genome copies of either template In vitro using conventional and multiplex PCR conditions. Under the multiplex conditions, co-amplification of P-globin and HBsAg DNA had a resultant effect on assay sensitivity. This study further highlights the importance of molecular diagnosis in HBV infectious individuals. If fully optimised, this assay could provide a possible diagnostic complement to serological detection in developing countries.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Adeyemi OO, Herod MR, Oladiji F, Fakunle YM, Babatunde AS, Agbede OO. A multi-template multiplex PCR assay for hepatitis B virus and human β-globin. J Med Virol. 2017; 1–8. https://doi.org/10.1002/jmv.24877 ; which has been published in final form at https://doi.org/10.1002/jmv.24877. This article may be used for non-commercial purposes in accordance with the Wiley Terms and Conditions for Self-Archiving. |
Keywords: | Hepatitis B,; HBs gene; Multiplex Polymerase Chain Reaction; Housekeeping Genes; Molecular diagnosis; Sensitivity |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Molecular Virology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Jun 2017 09:10 |
Last Modified: | 17 Jul 2018 00:38 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/jmv.24877 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117353 |